Plant Tissue Cuture & Biotechnology Conference 2008
April 11-13, 2008
Venue: Department of Botany, University of Dhaka

  Theme :
Opportunities and Challenges of Agricultural Biotechnology in Developing Countries

Inaugural Session

April 11, 2008 (Friday)

08:00 - 09:15 : Registration
09:15 - 09:30 : Guests take their seats
09:30 - 09:35 : Recitation from the Holy Qur'an
09:35 - 09:40 : Address of welcome and Introduction of foreign delegates
                        Prof. Dr. M. Imdadul Hoque
                        General Secretary, BAPTC&B
09:40 - 10:10 : Keynote address: Potentials of Biotechnology in Bangladesh Agriculture
                        Dr. Md. Abdur Razzaque , Executive Chairman, BARC
10:10 - 10:30 : Address by the Special Guest: Prof. Dr. Anwar Hossain
                        Dean, Faculty of Biological Sciences, Dhaka University
10:30 - 10:45 : Address by the Chief Guest: Professor Nazrul Islam
                        Chairman, Bangladesh University Grants Commission
10:45 - 10:50 : Presidential Address and Vote of Thanks: Dr. Md. Al-Amin
                        Vice President, BAPTC&B
10:50 - 11:00 : Refreshment

April 11, 2008 (Friday)

11:15 - 12:00 : Plenary Lecture I
                         Molecular Targeting and Drug Discovery in the post-Genomics Era

Speaker :         Dr. Zaheed Husain, Immune Disease Institute, Harvard Medical School,
                         Boston , MA ; Presently: Visiting Researcher, Institute of Biosciences,
                         University Putra Malaysia

Chairperson :   Prof. Ahmed A. Azad, FTWAS, FIAS, MASSAf, FRSSA, BAS,
                         Professor of Medical Biotechnology, University of Cape Town, South Africa,
                         TWAS Research Professor, BRAC University, Bangladesh

Co-Chairperson : Prof. Lutful Hassan, Dept. of Genetics & Pl. Breeding, BAU

Rapporteur : Dr. Aparna Islam, BRAC Univ. , Dhaka

Speaker : Dr. Abed Choudhury , CSIRO, Australia

Chairperson : Dr. M.C. Kharkwal, Principal Scientist, IARI, India

Co-Chairperson : Prof. A.K.M. Rafiul Islam, Dept. of Botany, Rajshahi University

Rapporteur : Dr. Md. Shamsher Ali, Biotech Div., BRRI

13:00 - 14:00 : LUNCH & PRAYER BREAK

14:00 - 14:30 : Opening of Poster session by

Prof. Ahmed A. Azad , FTWAS, FIAS, MASSAf, FRSSA, BAS,
                         Professor of Medical Biotechnology, University of Cape Town, South Africa, TWAS
                         Research Professor, BRAC University, Bangladesh

Title of the Poster

Sumeet Ranjan and Anjanii Kumar - In vitro Mass Propagation of Stevia reboudiana and Utilization of Steviosides as a Natural Sweetner (2)

Sambhavy and Anjanii Kumar - In vitro p ropagation of Coleus forskohlii Briq : An a ttempt to c onserve this e ndangered s pecies (3)

K.M.K Huda , M.E. Hoque and A. Khatun - In vitro Plant Regeneration Protocol of Tossa Jute ( Corchorus olitorius ) (4)

Deepak Kumar, Darakhshaan Akhtar Olayee and Anjanii Kumar - Mass Propagation of R. serpentina through Plant Tissue Culture Techniques (5)

P.I.P. Perera, V. Hocher, J.L. Verdeil, D.M.D. Yakandawala and L.K. Weerakoon - Effect of s elective s ubculturing on p lant r egeneration via a nther c ulture of c oconut (6)

S.M. Shahinul Islam, M.A. Bari and J.E. Schmid - The e ffect of d rought s tress p retreatment on a nther c ulture r esponse of w heat ( Triticum aestivum L.) (7)

Moumita Gangopadhyay and Sabita Bhattacharya - Hairy r oot c ultures of Plumbago indica for i ncreased p roduction of p lumbagin (12)

L.A. Banu, N. Nahar and M. A. Bari - Cell s uspension c ulture and e valuation of c ell g rowth in Abrus precatorious (13)

M.J. Hossain, M. Raman and M.A. Bari - Establishment of c ell s uspension c ulture and p lantlet r egeneration of Brinjal ( Solanum melongena L.) (14)

N. Nahar and M.A. Bari - In vitro c allogenesis and s pecific f eatures of c allus g rown from t hree c ultivars of b anana in Bangladesh (16)

F.M. Antara and M.A. Bari - Evaluation of Arsenic Hyperaccumulation of Fern Species in Bangladesh (17)

Animesh Biswas, M.A. Bari, Mohashweta Roy, S.K. Bhadra - In vitro p ropagation of Stemona tuberosa Lour., a r are m edicinal p lant of Bangladesh (18)

A.K.M.S. Howwan, F. Afroz, L.S. Bari, J.L. Munshi, M.A.A. Jahan and R. Khatun - Callus Induction and High Frequency Plant Regeneration of Scoparia dulcis Linn., a Perennial Medicinal Herb, through Axillary Shoot Proliferation (20)

Tanjina Akhtar Banu, R.H.Sarker and M.I. Haque - In vitro r egeneration and g enetic t ransformation in c hickpea ( Cicer aritinum ) (21)

F. Afroz and K.M. Nassiruddin - Transient GUS Expression in Papaya ( Carica papya L.) Explants after Infection by Agrobacterium tumefaciens (23)

Md. Mahmudul Hasan, A.N.M. Rubaiyath Bin Rahman and Asma Khatun - In vitro r egeneration and Agrobacterium -mediated t ransformation of Kenaf v arieties : t wo e fficient p rotocols for p lant d evelopment (24)

Md. Jaheduzzaman, Md. Ahsan Habib, Md. Salim Khan and A.N.M. Rubaiyath Bin Rahman - In vitro r egeneration of Centella asiatica (L.) Urban - a n i mportant m edicinal p lant (25)

N. Islam, M.A. Hossain and M.A.S. Miah - Low c ost b iotechnology for p roduction and u tilization of Stevia and Mushroom as f unctional f ood (27)

Sharmin Sultana and Sheikh Shamimul Alam - Karyotype Analysis in Three Morphological Forms of Lasia spinos (L.) Thwaites (Araceae) (29)

M.N. Huda, M. Zakaria, M.M. Hossain and M.A. Haque - In vitro Propagation of Asparagus ( Asparagus officinalis ) (30)

Md. Ashraful Haque, Toshihisa Kotake and Yoichi Tsumuraya - Elucidation of the s tructure of Chlorella g alactan- p rotein (31)

Sabina Yesmin and Shamima Nasrin - In vitro r egeneration of t wo v arieties of p otato ( Solanum tuberosum L.) (34)

Abu Ashfaqur Sajib, Md. Shahidul Islam, Md. Shamim Reza, Arpita Bhowmik, Layla Fatema and Haseena Khan - Tissue Culture Independent Transformation Protocol for Jute (37)

G.M. Al-Amin, M.I. Hoque and R.H. Sarker - Agrobacterium -mediated g enetic t ransformation of w hite j ute ( Corchorus capsularis L.) (41)

A. Ferdousi, M. S. Rahman, R. Alam, S. Rahman, Dante Adorada, G. Gregorio and Z. I. Seraj - Validation of DNA Markers from the Saltol Region in Specific Breeding Populations (42)

H.B. Shozib, M.J. Thomson, M.A. Salam, A.M. Ismail and Z.I. Seraj - Identification of Salt Tolerance QTLs in the Rice Landrace, Boilam and their Introgression into Farmer Popular Varieties (43)

Bivas Kumar Sarkar, M.I. Hoque and R.H. Sarker - In vitro r egeneration and Agrobacterium - mediated g enetic t ransformation of w hite j ute ( Corchorus capsularis L.) (48)

Md. Niamul Kabir, M.I. Hoque and R.H. Sarkar - Preliminary s tudies on the d evelopment of f ungal d isease r esistant p eanut ( Arachis hypogaea L.) t hrough g enetic t ransformation (49)

M. I. Hoque, R.H. Sarker and R. A. Sharmin - Development of f ungal r esistant Chickpea ( Cicer arietinum ) v arieties (50)

M. I. Hoque, R.H. Sarker and Surupa Das - Development of f ungal r esistant Lentil ( Lens culinaris Medik) v arieties (51)

Kazi Tariqul Islam , Md. Nurul. Islam, M.I. Hoque and R.H. Sarker - Agrobacterium -mediated g enetic t ransformation of Mungbean ( Vigna radiata (L.) Wilczek) (53)

R.H. Sarker, Subroto Kumar Das, M.I. Hoque - In vitro flowering and seed formation from transgenic shoots of lentil ( Lens culinaris Medik) following Agrobacterium -mediated genetic transformation (54)

M.M. Rashid and M.G. Rabbani - Molecular characterization of 24 sponge gourd germplasm using rapd (56)

A.K.M. Mohiuddin, Nilufer Hye Karim, Shahanaz Sultana and Zannatul Ferdous - Recovery from Initial Albinism of Anther Culture Derived Plantlets of Indica Rice ( Oryza sativa L.) (58)

S. T. Islam, M. E. Hoque, M. S. Hossain and A. Khatun - Genetic t ransformation in w hite j ute ( Corchorus capsularis ) (60)

M. A. Alam, M. A. Haque, M. R. Hossain and L. Hassan - Haploid Plantlet Regeneration Through Anther Culture of Five Oilseed Brassica Species (62)

M.A. Alam, M.R. Hossain, M.A. Haque and L. Hassan - Optimization of Callus Induction and Plantlet Regeneration Through Filament Culture of Five Oilseed Brassica Species (65)

S.B. Shahid, M.A. Quddus and L. Hassan - In vitro Regeneration Potentialities of Different Varieties of Cabbage and Cauliflower (67)

R. Rahman, M. A. Quddus and L. Hassan - Filament Culture of Oilseed Brassica in B5 Medium (69)

M.Z. Islam, L. Rahman and L. Hassan - Optimization of Regeneration Protocol of Ginger ( Zingiber officinale Rosc.) t hrough Meristem Culture (70)

M.S. Kabir, M.A. Quddus and L. Hassan - In vitro Regeneration of Garlic ( Allium sativum L.) (71)

Pinaki Sinha, M. Lokman Hakim and M. Firoz Alam - In vitro Clonal Propagation of Rhynchostylis retusa from Nodal Segments of Mature Plant (73)

Md. Tabiur Rahman, Md. Raihan Ali and Md. Abdul Matin - In vitro Micropropagation of Teak ( Tectona grandis L.) from Nodal Explant (74)

Shafiuddin Ahmed, Md. Raihan Ali and S.M. Mahbubur Rahman - Callus i nduction and s hoot r egeneration in Pointed gourd ( Trichosanthes dioca Roxb.) (75)

14:30 - 15:30 : Plenary Lecture III
                         Mutation Breeding and Food Security: Achievements and Prospects  (89)

Speaker : Dr. M.C. Kharkwal , Principal Scientist, IARI, India

Chairperson : Dr. Abed Choudhury, CSIRO, Australia 

Co-Chairperson : Dr. L. Hakim, IFRB, AERE, Savar, Dhaka 

Rapporteur : Prof. Md. Shahidul Haque, BAU, Mymensingh

15:30 - 15:45 : TEA BREAK 

15:45 - 18:00 : Scientific Session I
                         Production and conservation of medicinal, horticultural, ornamental,
                         timber and other cash crops through in vitro techniques

Chairperson : Prof. T.B. Jha , Dept. of Botany (PG & UG) Presidency College , University of Calcutta , India

Co-Chairperson : Prof. Dr. Md. Nurul Amin, Dept. of Botany, Rajshahi University

Rapporteur : Dr. Iftekhar Ahmad, Shahjalal University of Science & Technology, Sylhet

Speakers :

M.J. Alam, M.A. Mamun and I. Ahmad - In vitro Propagation of Pointed Gourd (1)

S.A.M. Nurul Islam, Saiful Alam M. Tareq, Haradhan Banik and Mahbubur Rahman - Cloning of Three Medicinal Plant Species ( Holarrhena antidysenterca Wall., Wedelia chinensis (Osb.)Merr. and Woodfordia fruticosa (L.) Kurz.) through in vitro Culture (8)

S. Gantait , N. Mandal, S. Bhattacharyya1 and P. K. Das - Sustained and a ccelerated i n vitro m ass m ultiplication with p ure g enetic i dentity in a nthurium (11)

K.K. Paul , M.A. Bari and Abul Mandal - In vitro r egeneration p rotocol e stablished in Elephant y am, Amorphophallus campanulatus Blume - a m edicinal a roid (15)

Animesh Biswas, M.A. Bari, Mohashweta Roy and S. K. Bhadra - In vitro p ropagation of Plumbago indica L. - a r are m edicinal p lant t hrough d irect o rganogenesis in n odal e xplants (19)

M. Khalekuzzaman and M.H. Rashid - Rapid Propagation of Watermelon ( Citrullus lanatus Thumb . ) From Field Grown Plants (52)

M.A. Alam, A.H.M. Kamal, N. Pervin, M.A. Prodhan and L. Hassan - Anther and Filament Culture in Oilseed Brassica Species (61)

N. Pervin, M.A. Alam, A.H.M. Kamal, M.A. Haque and L. Hassan - Standerdization of High Frequency Regeneration of Existing Genotypes of Jute ( Corchorus olitorius ) in Bangladesh (63)

N. Pervin , M.A. Alam, A.H.M. Kamal, M. A. Haque and L. Hassan - Standerdization of High Frequency Regeneration of Existing Genotypes of Jute ( Corchorus capsularis ) in Bangladesh (64)

K. Hossain, M. S. Ali and L. Hassan - In vitro Response of BRRI Released Rice Varieties on Different Culture Media (66)

Shyamal K. Roy and M. Azizur Rahman - In vitro Clonal Propagation of Two Bamboo Species, Bambusa vulgaris and Dendrocalamus giganteus (72)

Most. Mosfeqa Zahan, Shahina Islam, Rezaul Karim Mondal, Miskat Ara Akther Jahan, Salim Khan, Ahashan Habib, Shahina Akter, Tanjina Akhtar - Mass p ropagation of Feronia limonia L. (Kothbel) t hrough ti ssue c ulture (78)

Jesmin Akter, M.S. Haque and M. Abdul Karim - Plant Regeneration in Sweet Gourd (82)

April 12, 2008 (Saturday)

09:00 - 10:45 : Scientific session II
                         Biotechnology in Pharmaceutical Industries

Chairperson : Dr. Zaheed Husain , Immune Disease Institute, Harvard Medical School,
                        Boston , MA

Co-Chairperson : Dr. FH Ansarey , Executive Director, ACI Ltd.

Rapporteur : Dr. Al-Forkan, Dept. of Genetic Engineering & Biotech, CU

Keynote Paper : Strengthening of Molecular Biosciences Research Capacity and the Establishment of a Research-led Biopharmaceutical Industry will Foster Sustainable Development in Bangladesh

Speaker : Ahmed A Azad , FTWAS, FIAS, MASSAf, FRSSA, BAS ,
                  Professor of Medical Biotechnology, University of Cape Town, South Africa, TWAS
                  Research Professor, BRAC University, Bangladesh

                  Prof. Dr. Swapan Kumar Datta , TATA Innovation Fellow, Rash Behari Ghosh
                  Professor, University of Calcutta , India

                  Designer plant for drug discovery and human welfare (90)

                  Expression of peroxisome proliferator-activated receptor gamma and the growth
                  inhibitory effect of its synthetic ligands in human salivary gland cancer cell lines (59).

10:45 - 11:00 : TEA BREAK

11:00 - 13:00 :  Scientific Session III
                         Plant Transformation : Status and prospect of transgenic crops in
                         developing countries

Chairperson : Prof. S.K. Datta, Calcutta University , India

Co-Chairperson : Prof. Zeba Islam Seraj, Dept. Biochemistry & Mol. Biol., DU

Rapporteur : Prof. Md. Raihan Ali, Biotechnology Discipline, Khulna University

Speakers :

Md. Amzad Hossain, Hossain Md. Faruquee, Nadira Islam and A.N.M. Rubaiyath Bin Rahman -Agrobacterium -mediated Transformation of PsCBL and PsCIPK Genes for Salt and Drought Tolerance in Sugarcane ( Saccharum officinerum L.) (26)

M.A. Haque, T. Kotake and Y. Tsumuraya - Action of b -glucuronidase from Aspergillus niger on arabinogalactan-protein (AGP) (32)

R.H. Sarker, Sabina Yesmin and M.I. Hoque - Improvement of Eggplant ( Solanum melongena L.) through Agrobacterium - mediated transformation (33)

R.S. Tammi, S.M. Touhidul Islam, L.A. Lisa, S.L. Singla-Pareek and Z.I. Seraj - Agrobacterium- mediated t ransformation of OsNHX1 and PgNHX1 in Binnatoa under c onstitutive Actin1D and CaMV p romoters (45)

R. Malo, L.A. Lisa, S. Jahan, L. Parvin, F. Naznin, M. Amin, R.S. Tammi, N.M. Rasul, M. Nasiruddin, S. L. Singla-Pareek, N. Tuteja, R.H. Sarker and Z. I. Seraj - Rice Transformation for Salt Stress Tolerance (46)

S. Basak, M. S. Ali and L. Hassan - Genetic Transformation in Indica Rice (68)

Shamsul H. Prodhan, K. Nakao, K. Nagamiya, T. Motohashi , S. Jesmin, A. Komamine and H. Morishima - Introduction of Catalase Gene kat E in Indica Rice Through Agrobacterium -mediated Transformation to Attain Salt Tolerant Rice (76)

fathi hassan, Heiko Kiesecker and Hans-Jorg Jacobsen - Improvement of Pea against fungal diseases by Expression Family 19 Chitinase from Streptomyces olivaceoviridis ATCC 11238 (80)

Mouful Nahar and Bernhard Huchzermeyer - Chlorophyll fluorescence responses of two bean varieties during salt stress (85)

13:00 - 14:00 : LUNCH BREAK

14:00 - 15:30 : Scientific Session IV

Biosafety and public acceptance of genetically modified plants

Chairperson : Prof. Dr. Naiyyum Choudhury, Secretary, Bangladesh Academy of Sciences & Ex-Chairman, Bangladesh Atomic Energy Commission

Co-Chairperson : Mr. Mohammad Qamar Munir, Joint Secretary (Dev), Ministry of Environment & Forests, GOB

Rapporteur : Dr. Md. Shamsher Ali, Head, Biotechnology Division, BRRI

Speakers:   Mr. Md. Solaiman Haider
                  Project Director, National Biosafety Framework (NBF), Project, Department of
                  Environment, Ministry of Environment & Forests

                  : Highlights on the National Biosafety Framework and Biosafety Guidelines of
                  Bangladesh

                  : Prof. Dr. R.H. Sarker, Department of Botany, University of Dhaka

                  : Prof. Dr. Emdadul Haque Chowdhury,
                  Department of Pathology , Bangladesh Agricultural University, Mymensingh

15:30 - 15:45 : TEA BREAK

15:45 - 17:30 : Scientific Session V
                        Progress and prospect of commercialization of in vitro derived plants and
                        value added products

Chairperson : Dr. Md. Abdur Razzaque, Executive Chairman, BARC

Co-Chairperson : Mr. Mahbub Anam, Managing Director, Lal Teer Seeds (Pvt), Ltd.

Rapporteur : Dr. Md. Abdur Razzque Shah, BRAC Biotech., Joydebpur, Gazipur

Speakers :

: Mr. Mahbub Anam, Managing Director, Lal Teer Seed Limited

: Prof. Dr. M. Monzur Hossain, Department of Botany, Rajshahi University , Rajshahi

: Development of Commercial Plant Tissue Culture in Bangladesh

K.M. Formuzul Haque, Shamima Yesmin Chy, Shahina Akter, M. Abdul Wahab, K.K. Nath and Habibur Rahman Vuiyah - Collection, Identification and Biochemical Analyses of Different Sea Weeds (Marine Algae) from Saint Martin's Island (10)

Presentation by the Representatives of Private t issue Culture Laboratories of Bangladesh on: Achievements and limitations of commercialization of plant tissue culture in Bangladesh

M.A. Momin, N. Nahar and M. Hossain - Seed p otato p roduction through t issue c ulture by a man s eeds - A Sister Concern of a man g roup : Prospect and p roblems (59)

April 13, 2008 (Sunday)

09:00 - 11:00 : Scientific session VI
                        Tools for crop improvement: Marker assisted breeding, molecular
                        diagnostics

Chairperson : Dr. M. Serajul Islam, Ex-Member , Bangladesh Atomic Energy Commission

Co-Chairperson : Prof. M. Monzur Hossain, Dept. of Botany, RU 

Rapporteur : Dr. Mirza Mofazzal Islam, BINA, Mymensingh 

Speakers :

M. A. Mazid, M. A. Haque, H. U Ahmed, M. A. Salam, D. Mackill and A. M. Ismail - Biotechnology in a ction: Performance of Sub 1 l ines in f armer's f ields (88)

M. Salim Khan and H.-P. Muehlbach - Detection of s ome i mportant p otato v iruses and v iroid through m ultiplex-RT-PCR (22)

M.A. Hossain, N. Islam and M.A.S. Miah - Genetic d iversity in Bangladeshi and e xotic s ugarcane v arieties b ased on m icrosatellite m arkers (28)

Shohana Huq, Hosne Ara , Md. Abul Hassan and Sheikh Shamimul Alam - Comparative Karyotype Analysis of Typhonium trilobatum and its two Morphological Forms (35)

Zinnatun Nabi, Muhammad Shoyaib, Shakhinur Islam Mondal, Md. Maksudul Alam, Md. Shahidul Islam, Sazia Sharmin Sejuti, Sarmah Bin Nayeem and Haseena Khan - Identification of a Leucine-rich r epeat t ransmembrane p rotein Kinase g ene in Jute (36)

Md. Maksudul Alam, Rozalynne Samira, Mahdi Md. Mosa, Md. Ashraful Islam Bhuuiya and Haseena Khan - Bioinformatic Analysis of Genomic DNA of Jute (38)

Minhaz Uddin Pahloan, Shamima Islam Keka, Jesmine Akhter, Saaimatul Huq, Shams Zaman and Haseena Khan - Jute SSR m arkers : f or MAS and g enome m apping (39)

Samiul Haque, Nadim Ashraf, Aleya Awal, Saaimatul Huq, Md. Maksudul Alam, Nazlee Sharmin, Md. Shamim Reza and Haseena Khan - From Markers to Genes : Identification and Characterization of an Expressed Sequence Linked to low Temperature Tolerant in Jute (40)

M.S. Rahman, M.R. Islam, S. Rahman, A. Ferdousi, R. Malo, M. J. Thomson, M. A. Salam, A. M. Ismail and Z. I. Seraj - Introgression of ‘ Saltol' QTL into Bangladeshi m ega r ice v ariety BR11 and BRRI dhan28 t hrough m arker a ssisted b ackcrossing (44)

Salim Ahmed, Zinnatun Nabi, Muhammad Shoyaib, Shakhinur Islam Mondal, Md. Maksudul Alam and Haseena Khan - Identification and Confirmation of Jute Genes of Agronomical Importance (47)

D. Joyanti and M.G. Rabbani - Molecular Characterization of 48 Cucumber Germplasm Using RAPD (55)

M. Saifullah and M.G. Rabbani - Studies on genetic diversity of okra using morphological, biochemical and molecular markers (57)

H. Sohrawardy, M.M. Islam and S.N. Begum - Identification of Salt Tolerant Rice Lines in F3 Population of PNR-519× IR-29 Using SSR Markers (81)

K. Kibria, S. N. Begum and M. M. Islam - Genetic Diversity in Advanced Aromatic Rice Lines Using Ssr Markers (83)

Md. Mehedi Hasan­, Rokeya Begum, Md. Sazzadur Rahman and Zeba I. Seraj - Variability b etween Hori dhan and BR11: What is their m olecular r elationship ? (84)

Biswanath Sikdar, Moimee Bhattacharya, Biplab Ghosh, Ashutosh Mukherjee, Anuradha Banerjee, Enakshi Ghosh, S.C. Roy - Study of g enetic d iversity in s ome e conomically i mportant m embers of Cucurbitaceae u sing i sozyme, RAPD and ISSR m arkers (86)

11:00 - 11:15 : Tea Break

11:15 - 13:00 : Concluding session and Adoption of Recommendations

13:00 - 14:00 : LUNCH

14:00 : CLOSING OF THE CONFERENCE