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Plant Tissue Cult. 5(2) : 95-103, 1995
Androgenesis in Anther Cultures of an indica Rice cv. IR43
Himani Pande
Department of Botany, University of Delhi, Delhi 110 007, India
Key words:
Androgenesis, indica rice, Heat shock, Colchicine, Green plants
Abstract
Effects of various parameters on the frequency of pollen embryogenesis in anther cultures of Oryza sativa ssp. indica cv. IR43 were studied. A combination of centrifugation (1000 rpm for 5 min) and cold treatment (100C for 10 days) of the anthers enhanced androgenic response fivefold over the control. Heat shock (450C for 5 min) and colchicine (50 mg/1) treatments also promoted androgenesis but were inferior to that of centrifugation. The frequency of green plants produced was distinctly higher in the first two treatments.
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