Plant Tissue Cult. 5(2) : 95-103, 1995
Androgenesis in Anther Cultures of an indica Rice cv. IR43
Department of Botany, University of Delhi, Delhi 110 007, India
Androgenesis, indica rice, Heat shock, Colchicine, Green plants
Effects of various parameters on the frequency of pollen embryogenesis in anther cultures of Oryza sativa ssp. indica cv. IR43 were studied. A combination of centrifugation (1000 rpm for 5 min) and cold treatment (100C for 10 days) of the anthers enhanced androgenic response fivefold over the control. Heat shock (450C for 5 min) and colchicine (50 mg/1) treatments also promoted androgenesis but were inferior to that of centrifugation. The frequency of green plants produced was distinctly higher in the first two treatments.